sheep anti foxc2  (R&D Systems)

Journal: Development (Cambridge, England)

Article Title: TIE1 dependent lymphatic vascular remodeling is mediated by its second tyrosine kinase domain.

doi: 10.1242/dev.204469

Figure Lengend Snippet: Fig.10. FOXC2 expression was abolished in Tie1 deficient lymphatics but restored

Article Snippet: Primary antibodies for immunohistochemistry Antibody Supplier Catalogue # Application Goat anti-mouse VEGFR3 R&D Systems AF743 1:50 Goat anti-human TIE1 R&D Systems AF619 1:100 Goat anti-mouse TIE2 R&D Systems AF762 1:200 Goat anti-mouse integrinα9 R&D Systems AF3827 1:50 Goat anti-VE-Cadherin R&D Systems AF1002 1:400 Goat anti-human Prox1 R&D Systems AF2727 1:100 Sheep anti-mouse FOXC2 R&D Systems AF6989 1:200 Rabbit anti-GFP Invitrogen A11122 1:200 Rabbit anti-FOXO1 Cell Signaling 2880 1:200 Rabbit anti-Lyve1 abcam Ab14917 1:800 Rabbit anti-Prox1 abcam Ab37128 1:250 Rat anti-mouse CD31 PD Pharmingen 557355 1:200 Rat anti-endomucin abcam ab106100 1:200 Development: doi:10.1242/dev.204469: Supplementary information D ev el o pm en t • S up pl em en ta ry in fo rm at io n

Techniques: Expressing

Journal: Investigative Ophthalmology & Visual Science

Article Title: Primary Cilium in Neural Crest Cells Crucial for Anterior Segment Development and Corneal Avascularity

doi: 10.1167/iovs.65.3.30

Figure Lengend Snippet: Loss of primary cilia by Ift46 deficiency in NCCs induces the decrease of Shh signal activity, the increase of cell proliferation, and the persistent expression of Foxc1 and Foxc2 in the corneal mesenchyme. ( A ) Decrease of Shh signal activity in the periocular mesenchyme (POM) of NC- Ift46 F/F ;Gli1 lacZ/+ mice. Shh activity was assessed by Gli1-LacZ expression in the POM of Ift46 F/F ;Gli1 lacZ/+ and NC- Ift46 F/F ;Gli1 lacZ/+ mice at E12.5 and E13.5. Ift46 F/F ;Gli1 lacZ/+ mice displayed Gli1-LacZ expression in the POM, whereas NC- Ift46 F/F ;Gli1 lacZ/+ mice exhibited a significant reduction in Gli1-LacZ expression in the POM, indicating a decrease in Shh signal activity. ( B ) Persistent expression of ocular NCC markers Foxc1 and Foxc2 in the corneal mesenchyme of NC- Ift46 F/F mice at E12.5 and E13.5. The corneas from Ift46 F/F and NC- Ift46 F/F mice were stained with Foxc1, Foxc2, and Pitx2 antibodies. At E12.5, both Ift46 F/F and NC- Ift46 F/F mice displayed Foxc1 and Foxc2 expression in the POM and corneal mesenchyme. By E13.5, expression was restricted to the POM in Ift46 F/F mice, whereas in NC- Ift46 F/F mice, Foxc1 and Foxc2 were ectopically expressed in increased amounts in the corneal mesenchyme. Pitx2 expression remained consistent in both the periocular and corneal mesenchyme. ( C ) Hyperproliferation in the corneal stroma in NC- Ift46 F/F mice. Cell proliferation in the corneal stroma of Ift46 F/F and NC- Ift46 F/F mice at E13.5, E15.5 and E17.5 was examined using Cyclin D1 staining. Persistent cell proliferation was observed in the corneal stroma of NC- Ift46 F/F mice throughout ocular development. ( D ) Quantification of cell proliferation in the corneal stroma of Ift46 F/F and NC- Ift46 F/F mice at E13.5 and E15.5 through Cyclin D1 staining. cm, corneal mesenchyme; pom, periocular mesenchyme. *** P < 0.001, Student's t -test. Scale bars : 100 µm.

Article Snippet: The primary antibodies used included mouse anti-N-cadherin (clone 13A9, 1:100; Upstate Biotechnology, Charlottesville, VA, USA), mouse anti-α smooth muscle actin FITC conjugated (1:500; Sigma-Aldrich Corp.), goat anti-mouse LYVE1 (1:100; R&D systems, Minneapolis, MN, USA), PE-conjugated anti-mouse CD31 (PECAM-1, 1:200; BD Pharmingen), rabbit anti-Cyclin D1 (1:100; Abcam), rabbit anti-FOXC1 (1:100; Abcam), sheep anti-mouse FoxC2 (1:100; R&D systems), sheep anti-hPITX2 (1:100; R&D systems), rabbit anti-PAX6 (1:100; Abcam), mouse anti-cytokeratin 14 (1:100, Abcam), and rabbit anti-ARL13B (1:100; Proteintech, Rosemont, IL, USA).

Techniques: Activity Assay, Expressing, Staining

Journal: Development (Cambridge, England)

Article Title: A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors

doi: 10.1242/dev.201229

Figure Lengend Snippet: Transcriptional profiles of cardiac mesoderm in Mesp1 KO embryos. (A,F,K) scRNA-seq UMAP atlases of Ai14, Smarcd3 -F6 double-positive mesoderm cells from early (A; 5504 cells), middle (F; 7666 cells) and late (K; 22,622 cells) developmental embryo stages. (B,C,G,H,L,M) Associated UMAPs colored by genotype (B,G,L) and Smarcd3 -F6-eGFP expression (C,H,M). (D,E) Early mesoderm DGE in LSMeso2 (D) and EomesPSMeso (E). (I,J) Middle mesoderm DGE in LSMeso2 (I) and Mesp1ME (J). (N-P) Late mesoderm DGE in Meso2 (N), Meso1 (O), and postLPM (P). Significant changes denoted with adjusted P <0.05. antPrSoM, anterior presomitic mesoderm; DE-ME, definitive endoderm/mesendoderm; Foxc2M, Foxc2 + mesoderm; Meso, mesoderm; PSMeso, primitive streak mesoderm; PS-NM, primitive streak/neuromesodermal.

Article Snippet: Antibodies used in this study were: sheep polyclonal Foxc2 (R&D Systems, AF6989), chicken polyclonal GFP (Aves Labs, GFP-1020), rabbit polyclonal Cre (Millipore, 69050).

Techniques: Expressing

Journal: bioRxiv

Article Title: A Mesp1 -dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors

doi: 10.1101/2022.08.22.504863

Figure Lengend Snippet: (A) Overlay of Mesp1 , Foxc2 , and Ai14 Mesp1 -lineage gene expression in cell types of Middle mesoderm atlas UMAP. (B) Immunostaining and Light Sheet Confocal microscopy for Foxc2 (magenta), Smarcd3 -F6 (green) and Mesp1 via Cre detection (blue) in Middle stage embryos (∼E6.75). Arrowheads denote domain boundaries in control and disruption in Mesp1 KO embryo. Scale bars are 100 μm.

Article Snippet: Antibodies used in this study: sheep polyclonal Foxc2 (R&D, AF6989), chicken polyclonal GFP (Aves, GFP-1020), rabbit polyclonal Cre (Millipore, 69050).

Techniques: Gene Expression, Immunostaining, Confocal Microscopy, Control, Disruption